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Home >> Antibodies >> Double Stranded DNA (dsDNA) (Nuclear Marker)

Double Stranded DNA (dsDNA) (Nuclear Marker)

Mouse Monoclonal Antibody [Clone 121-3]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Mouse Monoclonal Antibody (121-3)

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Formalin-fixed, paraffin-embedded human Tonsil stained with Double Stranded DNA Mouse Monoclonal Antibody (121-3)

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Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells with Double Stranded DNA Mouse Monoclonal Antibody (121-3)followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

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SDS-PAGE Analysis Purified Double Stranded DNA Mouse Monoclonal Antibody (121-3). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Not Applicable

Positive Control

Raji, Jurkat or HeLa cells. Tonsil or Colon.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of Burkitt's cells

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG3, kappa

Mol. Weight of Antigen

Not Known

Specificity & Comments

This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes; DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.

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200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.

Bioinformatics

NBT00945