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Home >> Antibodies >> DNA Ligase 1

DNA Ligase 1

Mouse Monoclonal Antibody [Clone 1A9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

Positive Control

MOLT-4, PC-3 or Jurkat cell lysates.

Known Applications & Suggested Dilutions

Western Blot, ELISA, IP         More Details

Western Blot (1-2ug/ml)
ELISA
Immunoprecipitation
Optimal dilution for a specific application should be determined.

Immunogen

Full-length native cow LIG1 protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Mouse. Rat. Bovine.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

133kDa

Specificity & Comments

DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Barnes, D.E., et al. 1990. Human DNA Ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 87: 6679-6683.

Bioinformatics

  •  Entrez Gene ID: 3978
  •  Gene Symbol: LIG1
  •  SwissProt: P18858
  •  Chromosome Location: 19q13.33
  •  Unigene: 1770
NBT02177