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DNA Ligase 1 Antibody [1A9]

In Stock
Catalog Number Formulation Size Price
3978-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3978-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3978-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Western Blot (WB)
2-4ug/ml

Summary

DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5′ phosphoryl and 3′ hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Molt-4, PC-3 or Jurkat cell lysates.
Immunogen
Full-length native cow LIG1 protein
Alternate Names
DNA ligase 1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1, DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
1A9
Chromosome Location
19q13.33
Mol. Weight of Antigen
133kDa

Functions

  • DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Key References

  • Barnes, D.E., et al. 1990. Human DNA Ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 87: 6679-6683.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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