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Home >> Antibodies >> Cytokeratin, Multi

Cytokeratin, Multi

Mouse Monoclonal Antibody [Clone SPM583]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded colon (10X) stained with Multi Keratin Monoclonal Antibody (SPM583).

Product Details

Positive Control

A431 cells, Skin, Colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-4ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Keratin-enriched preparation from cultured human A431

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Cow. Rat. Mouse. Guinea Pig. Frog. Goat. Marmoset. Pig.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

Multiple

Specificity & Comments

This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bartek J et. al. J Pathol, 1991, 164(3):215-24.
  2. Bartkova J; et al. Neoplasma, 1991, 38:439-46.
  3. Lane EB; Alexander CM. Seminars in Cancer Biology, 1990, 1:165-79.
  4. Kasper M. Histochemistry, 1991, 95(6):613-20.

Bioinformatics

NBT00393