Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 8 Mouse Monoclonal Antibody (SPM538)
Immunofluorescent staining of MCF-7 cells using Cytokeratin 8 Mouse Monoclonal Antibody (SPM538) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot. (Red).
Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 8 Mouse Monoclonal Antibody (SPM538) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
SDS-PAGE Analysis Purified Cytokeratin 8 Mouse Monoclonal Antibody (SPM538). Confirmation of Integrity and Purity of Antibody
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.