Home >> Antibodies >> Cytokeratin 8 (KRT8)

Cytokeratin 8 (KRT8)

Recombinant Rabbit Monoclonal Antibody [Clone KRT8/6472R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody (KRT8/6472R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT8/6472R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified KRT8 Recombinant Rabbit Monoclonal Antibody (KRT8/6472R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human colon stained with Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody (KRT8/6472R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using KRT8/6472R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified KRT8 Recombinant Rabbit Monoclonal Antibody (KRT8/6472R). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; Type-II Keratin Kb8

Positive Control

HCT116, MCF-7 or A431 cells. Human skin, colon, lung or breast carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human KRT8 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

52.5kDa

Specificity & Comments

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.