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Home >> Antibodies >> CREB5 / CRE-BPa

CREB5 / CRE-BPa

Mouse Monoclonal Antibody [Clone PCRP-CREB5-1G8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow cytometric analysis of PFA-fixed HeLa cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescent analysis of PFA-fixed K562 cells stained with CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

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Flow cytometric analysis of PFA-fixed K562 cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Western blot analysis of HeLa cell lysate using CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8).

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SDS-PAGE Analysis. Purified CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

cAMP response element-binding protein CREBPa (CRE-BPa); cAMP responsive element binding protein 5 isoform alpha; CRE BPA; CREBPA; cyclic AMP responsive element binding protein 5

Positive Control

HeLa, U87 or K562 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CREB5 protein

Cellular Localization

Nucleus.

Species Reactivity

Human. Predicted to work with Mouse and Rat.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

57kDa

Specificity & Comments

The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Chen, T., et al. 2018. Virus Genes 54: 514-526
  2. Lian, H., et al. 2018. Nat. Commun. 9: 3349.

Bioinformatics

  •  Entrez Gene ID: 9586
  •  Gene Symbol: CREB5
  •  SwissProt: Q02930
  •  Chromosome Location: 7p15.1
  •  Unigene: 437075