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Learn about our comprehensive antibody validation methods to ensure monospecificity. Antibody Validation>>
Flow cytometric analysis of PFA-fixed HeLa cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunofluorescent analysis of PFA-fixed K562 cells stained with CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
Flow cytometric analysis of PFA-fixed K562 cells. CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Western blot analysis of HeLa cell lysate using CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE Analysis. Purified CREB5 Mouse Monoclonal Antibody (PCRP-CREB5-1G8). Confirmation of Purity and Integrity of Antibody.
The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.
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