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Home >> Antibodies >> CD99 / MIC2 (Ewing's Sarcoma Marker)

CD99 / MIC2 (Ewing's Sarcoma Marker)

Mouse Monoclonal Antibody [Clone SPM586]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with CD99 Monoclonal Antibody (SPM586).

Product Details

Synonyms

12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Positive Control

Pancreas or Ewing's sarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (5-10ul/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at Room Temp)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes

Cellular Localization

Cell surface

Species Reactivity

Human. Rat.

Host / Ig Isotype

Mouse / IgM, kappa

Mol. Weight of Antigen

27-32kDa

Specificity & Comments

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sandrin MS, et. al. Immunogenetics, 1992, 35(4):283-5.

Bioinformatics

  •  Entrez Gene ID: 4267
  •  Gene Symbol: CD99
  •  SwissProt: P14209
  •  Chromosome Location: Xp22.33
  •  Unigene: 653349
NBT00869