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Recombinant CD99 / MIC2 (Ewing s Sarcoma Marker) Antibody [MIC2/3478R]

In Stock
Catalog Number Formulation Size Price
4267-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4267-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4267-RBM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD99 is a type I transmembrane glycoprotein encoded by the CD99 gene and the functions of CD99 in cells in which CD99 was highly expressed have been studied and they were as follows: cell death of thymocytes and T lymphocytes, migration through monocyte endothelial junctions by adhesion and diapedesis, cell-cell adhesion in lymphocytes, maintenance of cellular morphology in Hodgkin and Reed/Sternberg cells, and recruitment of T cells.CD99 expression has been reported in many cell types, such as hematopoietic cells, endothelial cells, central nervous system ependymal cells, thymocytes, granular cells of the ovary, Sertoli cells, and pancreatic islet cells. And in tumors it expressed by virtually almost all Ewing s sarcoma and primitive peripheral neuroectodermal tumors (ES/PNET) and demonstrates strong and diffuse membranous staining. Other tumors that may show CD99 expression include neuroendocrine carcinomas, mesenchymal chondrosarcomas, solitary fibrous tumors, synovial sarcomas, vascular tumors, small round blue cell tumors, lymphoblastic lymphoma, acute myeloid leukemia, and myeloid sarcoma. Studies have shown that CD99 may be a sensitive marker for Ewing s sarcoma and peripheral neuroectodermal tumors.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
MOLT-4 cells. Pancreas or Ewing s sarcoma
Immunogen
Synthetic peptide corresponding to CD99 residues within aa85 C terminal of CD99 was used as an immunogen
Alternate Names
CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2, 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MIC2/3478R
Chromosome Location
Xp22.33
Mol. Weight of Antigen
19kDa

Functions

  • Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Key References

  • Gawon Choi, et al. Journal of Pathology and Translational Medicine 2016; 50: 361-368.
  • Ventura S, et al. Oncogene. 2016 Jul 28;35(30):3944-54.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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