2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD74 (B-Cell Marker)

CD74 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone SPM523]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Ab (SPM523).

img

Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Mouse Monoclonal Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

img

Flow Cytometric Analysis of Human Raji cells using CD74 Mouse Monoclonal Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).

Product Details

Synonyms

CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Positive Control

Daudi cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

SU-DHL-4 lymphoma cells

Cellular Localization

Cell surface and paranuclear globular

Species Reactivity

Human. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

33-41kDa

Specificity & Comments

CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Epstein AL et. al. J of Immunology 133: 1028-1036, 1984.
  2. Marder RJ et. al. Lab Invest 52: 497-504, 1985.

Bioinformatics

  •  Entrez Gene ID: 972
  •  Gene Symbol: CD74
  •  SwissProt: P044233
  •  Chromosome Location: 5q33.1
  •  Unigene: 436568
NBT00056