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Home >> Antibodies >> CD74 (B-Cell Marker)

CD74 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone LN-2 + CLIP/813]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Antibody (LN-2 + CLIP/813).

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Flow Cytometric Analysis of Raji cells. CD74 Mouse Monoclonal Antibody (LN-2+CLIP/813) followed by goat anti-Mouse IgG-CF488. Orange: cells are not permeabilized. Blue: cells are permeabilized. Isotype Control (Red).

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Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Mouse Monoclonal Antibody (LN-2+ CLIP/813) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Positive Control

Daudi or Raji cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

SU-DHL-4 lymphoma cells (LN-2); Recombinant full-length human CD74 protein (CLIP/813)

Cellular Localization

Cell surface and paranuclear globular

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa + Mouse / IgG1, kappa

Mol. Weight of Antigen

33-41kDa

Specificity & Comments

It recognizes a protein of ~35kDa, identified as CD74. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Wilson, K.M., et al. 1993. Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. Immunology 79: 331-335.

Bioinformatics

  •  Entrez Gene ID: 972
  •  Gene Symbol: CD74
  •  SwissProt: P044233
  •  Chromosome Location: 5q33.1
  •  Unigene: 436568
NBT01207