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Home >> Antibodies >> CD55 / Decay Accelerating Factor (DAF)

CD55 / Decay Accelerating Factor (DAF)

Mouse Monoclonal Antibody [Clone F4-29D9]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Green). Counterstain is RedDot.

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Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

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SDS-PAGE Analysis Purified CD55 Mouse Monoclonal Antibody (F4-29D9) . Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

CD55; CD55 Cromer blood group system; Complement decay-accelerating factor; CROM; DAF; Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF

Positive Control

Jurkat, HUT-78, K562, YT, U937, MG63 and human lymphocytes. Human lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Human umbilical vein endothelial cells (HUVEC)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

Recognizes a single chain glycoprotein of 70kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, 1997.B.E. Loveland - in Leucocyte Typing VI - Part 6 - Known Non-Lineage CD Antigens - NL11 - CD55 Workshop Panel Report pp519-520, 1997

Bioinformatics

  •  Entrez Gene ID: 1604
  •  Gene Symbol: CD55
  •  SwissProt: P08174
  •  Chromosome Location: 1q32
  •  Unigene: 126517
NBT00986