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CD55 / Decay Accelerating Factor (DAF) Antibody [F4-29D9]

In Stock
Best Seller
Catalog Number Formulation Size Price
1604-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1604-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1604-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD55 / Decay Accelerating Factor (DAF) Antibody

Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Green). Counterstain is RedDot.

CD55 / Decay Accelerating Factor (DAF) Antibody - Image 2

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

CD55 / Decay Accelerating Factor (DAF) Antibody - Image 3

SDS-PAGE Analysis of Purified CD55 Mouse Monoclonal Antibody (F4-29D9) . Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a single chain glycoprotein of 70kDa, identified as CD55. This MAb was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Immunology, B Cell Markers, Complement System, Endothelial Cell Marker, Mast Cell Marker, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD55
Positive Control
HUT-78, Jurkat, K562, MG63 and human lymphocytes. Human lymph nodes and tonsils., U937, YT
Immunogen
Human umbilical vein endothelial cells (HUVEC)
Alternate Names
Complement decay-accelerating factor, CD55; CD55 Cromer blood group system; Complement decay-accelerating factor; CROM; DAF; Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form; GPI-DAF

Database Links

Entrez Gene ID
1604
SwissProt
P08174

Additional Information

Clone
F4-29D9
Chromosome Location
1q32
Mol. Weight of Antigen
70kDa

Related Antibodies

View All CD55 Antibodies

Functions

  • This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).
  • (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.
  • (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).
  • (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.

Key References

  • Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, 1997.B.E. Loveland - in Leucocyte Typing VI - Part 6 - Known Non-Lineage CD Antigens - NL11 - CD55 Workshop Panel Report pp519-520, 1997

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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