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Home >> Antibodies >> CD55 / Decay Accelerating Factor (DAF)

CD55 / Decay Accelerating Factor (DAF)

Mouse Monoclonal Antibody [Clone 143-30]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Immunofluorescent staining of PFA-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (143-30) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.

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Flow Cytometric Analysis of PFA-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (143-30) followed by goat anti-mouse IgG-CF488 (blue); Isotype Control (red)

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SDS-PAGE Analysis Purified CD55 Mouse Monoclonal Antibody (143-30). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

CD55; CD55 Cromer blood group system; Complement decay-accelerating factor; CROM; DAF; GPI-DAF; Decay Accelerating Factor for Complement; Decay accelerating factor GPI-form; Decay accelerating factor soluble-form

Positive Control

Jurkat cells or human lymphocytes. Human lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

PHA stimulated human PBL

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

70kDa

Specificity & Comments

Recognizes a single chain glycoprotein of 70kDa, identified as CD55/DAF. It is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 may be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kishimoto T. et al., eds. Leukocyte Typing VI, Garland Publishing, Inc, New York and London, 1997.Koretz, K. et al., Decay-accelerating factor (DAF, CD55) in normal colorectal mucosa, adenomas and carcinomas. British J. Cancer 66: 810-814, (1992).

Bioinformatics

  •  Entrez Gene ID: 1604
  •  Gene Symbol: CD55
  •  SwissProt: P08174
  •  Chromosome Location: 1q32
  •  Unigene: 126517