Flow Cytometric Analysis of Jurkat cells. CD3e Mouse Monoclonal Antibody (CRIS-7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Immunofluorescence Analysis of PFA-fixed Jurkat cells. CD3e Mouse Monoclonal Antibody (CRIS-7) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei stained with RedDot (red).
SDS-PAGE Analysis Purified CD3e Mouse Monoclonal Antibody (CRIS-7). Confirmation of Integrity and Purity of Antibody.
Flow cytometric analysis of lymphocyte-gated peripheral blood mononuclear cells. CD3e Mouse Monoclonal Antibody (CRIS-7) followed goat anti-mouse IgG-CF488A (green); unstained PBMCs (gray).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.