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Home >> Antibodies >> CD33 / SIGLEC3 (Myeloid Cell Surface Antigen)

CD33 / SIGLEC3 (Myeloid Cell Surface Antigen)

Recombinant Rabbit Monoclonal Antibody [Clone SIGLEC3/7046R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified CD33 Recombinant Rabbit Monoclonal Antibody (SIGLEC3/7046R). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

gp67; My9; Myeloid cell surface antigen CD33; p67; Sialic acid-binding Ig-like lectin 3 (SIGLEC3)

Positive Control

Human dendritic and mast cells. Human lymph nodes and tonsils.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa51-224) of human CD33 protein (exact sequence is proprietary)

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Favaloro EJ, et al. Dis Markers. 1987;5(4):215-25.
  2. Favaloro EJ, et al. Br J Haematol. 1988;69(2):163-71.

Bioinformatics

  •  Entrez Gene ID: 945
  •  Gene Symbol: CD33
  •  SwissProt: P20138
  •  Chromosome Location: 19q13.33
  •  Unigene: 83731