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Home >> Antibodies >> CD27 (Tumor Necrosis Factor Receptor Superfamily 7)

CD27 (Tumor Necrosis Factor Receptor Superfamily 7)

Mouse Monoclonal Antibody [Clone LPFS2/1611]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (LPFS2/1611). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Formalin-fixed, paraffin-embedded human Spleen stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Formalin-fixed, paraffin-embedded human Colon stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).

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Flow Cytometric Analysis of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescence staining of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27 Mouse Monoclonal Antibody (LPFS2/1611)

Product Details

Synonyms

LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7

Positive Control

Ramos cells. Human peripheral blood lymphocytes. Ramos and lymph node cell lysates. Human tonsil and stomach tissues.

Known Applications & Suggested Dilutions

ELISA, Flow Cyt, IF, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CD27 protein

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

120kDa

Specificity & Comments

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. De Rie MA, et al. Clin Exp Dermatol. 1996 Mar;21(2):104-11.

Bioinformatics

  •  Entrez Gene ID: 939
  •  Gene Symbol: CD27
  •  SwissProt: P26842
  •  Chromosome Location: 12p13
  •  Unigene: 355307
NBT01611