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Carrier-Free Available
Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).
SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (LPFS2/1611). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).
Formalin-fixed, paraffin-embedded human Spleen stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).
Formalin-fixed, paraffin-embedded human Colon stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).
Flow Cytometric Analysis of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence staining of Ramos cells using CD27 Mouse Monoclonal Antibody (LPFS2/1611) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD27 Mouse Monoclonal Antibody (LPFS2/1611) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.
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