Analysis of Protein Array containing more than 19,000 full-length human proteins using CD22 Mouse Monoclonal Antibody (BLCAM/1796)
Flow Cytometric Analysis of Ramos cells using CD22 Mouse Monoclonal Antibody (BLCAM/1796) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence staining of paraformaldehyde-fixed Ramos cells with CD22 Mouse Monoclonal Antibody (BLCAM/1796) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).
Western Blot Analysis of human Raji cell lysate using CD22 Mouse Monoclonal Antibody (BLCAM/1796).
SDS-PAGE Analysis Purified CD22 Mouse Monoclonal Antibody (BLCAM/1796). Confirmation of Integrity and Purity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.