Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/2637R). Inset: PBS instead of primary antibody, secondary control.

Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/2637R).

SDS-PAGE Analysis Purified CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/2637R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/2637R).

CD22 / BL-CAM (B-Cell Marker) [BLCAM/2637R]

Name: CD22 / BL-CAM (B-Cell Marker)
SKU: 933-RBM7
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cell membrane

Gene Name

CD22

Mol. Weight of Antigen

130-140kDa

Immunogen

Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Database Links

Entrez Gene ID

933

Unigene

579691

SwissProt

P20273

Additional Information

Clone

BLCAM/2637R

Human Gene Symbol

CD22

Chromosome Location

19q13.1

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Sato S et al. Semin Immunol 1998, 10:287-297.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.