Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone SPM494]

Printable

Key References

Bioinformatics

Catalog #

Size

0.1 ml

0.5 ml

Price (USD)

219.00

499

Formulation

Culture Supernatant

Performance Guarantee

Catalog No

Size

0.1 ml

0.5 ml

Formulation

Culture Supernatant

Price (USD)

219.00

499

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Monoclonal Antibody (SPM494)

Flow Cytometric Analysis of Raji cells using CD20 Monoclonal Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence staining of MOLT-4 cells using CD20 Monoclonal Antibody (SPM494) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Monoclonal Antibody (SPM494)

Flow Cytometric Analysis of Raji cells using CD20 Monoclonal Antibody (SPM494) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence staining of MOLT-4 cells using CD20 Monoclonal Antibody (SPM494) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Product Details

Synonyms

APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Positive Control

Daudi, Raji and U266 and human lymphocytes. Lymph nodes and tonsils.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P More Details

Flow Cytometry (2-5ul/million cells)
Immunofluorescence (1:100-1:200)
Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Human tonsil B cells

Cellular Localization

Predominantly cell surface with some cytoplasmic

Species Reactivity

Human. Baboon. Monkey. Does not react with Cow, Dog, Pig or Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.