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Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (MS4A1/3411).
SDS-PAGE Analysis of Purified CD20 Mouse Monoclonal Antibody (MS4A1/3411). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of Raji cells. CD20 Mouse Monoclonal Antibody (MS4A1/3411) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (MS4A1/3411)followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (MS4A1/3411).
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (MS4A1/3411).
Western Blot Analysis of Raji lysate using CD20 Mouse Monoclonal Antibody (MS4A1/3411).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
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