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SDS-PAGE Analysis Purified CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).
Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Inset: PBS instead of primary antibody; secondary only negative control.
IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine and Rabbit Intestine tissue lysates using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
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