2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Recombinant Mouse Monoclonal Antibody [Clone rIGEL/773]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

SDS-PAGE Analysis Purified CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773). Confirmation of Purity and Integrity of Antibody.

img

Flow Cytometric Analysis of Raji cells using CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

img

Immunofluorescence staining of Raji cells using CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD20 Monospecific Recombinant Mouse Monoclonal Antibody (rIGEL/773).

img

Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773).

Product Details

Synonyms

APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)

Positive Control

Daudi, Raji or U266 (FACS). Human lymphocytes. Lymph node and tonsil (IHC).

Known Applications & Suggested Dilutions

IF, Flow Cyt, IHC-P         More Details

Immunofluorescence (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MS4A1 fragment

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Related Products



200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tedder, T.F., et al. 1994. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol. Today 15: 450-454.

Bioinformatics

  •  Entrez Gene ID: 931
  •  Gene Symbol: MS4A1
  •  SwissProt: P11836
  •  Chromosome Location: 11q12.2
  •  Unigene: 712553
NBT01829