Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone MS4A1/7015R]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

SDS-PAGE Analysis Purified CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis Purified CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Recombinant Rabbit Monoclonal Antibody (MS4A1/7015R). Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using MS4A1/7015R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; MS4A1; MS4A2

Positive Control

Daudi, Raji or U266 cells. Human lymphocytes. Human lymph node or tonsil.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 97-297) corresponding to C-terminal of human CD20 protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human and Dog. Expected to react with Monkey, Baboon, Gorilla and Chimpanzee.

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.