Home >> Antibodies >> CD20 / MS4A1 (B-Cell Marker)

CD20 / MS4A1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone IGEL/773]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

Flow Cytometric Analysis of Raji cells .CD20 Mouse Monoclonal Antibody (L26); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (IGEL/773). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

Flow Cytometric Analysis of Raji cells .CD20 Mouse Monoclonal Antibody (L26); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (IGEL/773). Confirmation of Purity and Integrity of Antibody.

Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Product Details

Synonyms

APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; Leukocyte surface antigen Leu-16; Ly44; MS4A1; MS4A2

Positive Control

Daudi, Raji, and U266 cell lines. Human lymphocytes. Human lymph node or tonsil.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Immunohistology More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MS4A1 protein

Cellular Localization

Predominantly cell surface with some cytoplasmic

Species Reactivity

Human. Others not known.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

33-37kDa

Specificity & Comments

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.