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CD20 / MS4A1 (B-Cell Marker) Antibody [IGEL/773]

Additional information

Catalog Number: 931-MSM3
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Best Seller
Catalog Number Formulation Size Price
931-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
931-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
931-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD20 / MS4A1 (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

CD20 / MS4A1 (B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (IGEL/773).

CD20 / MS4A1 (B-Cell Marker) Antibody - Image 3

Flow Cytometric Analysis of Raji cells .CD20 Mouse Monoclonal Antibody (L26); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

CD20 / MS4A1 (B-Cell Marker) Antibody - Image 4

SDS-PAGE Analysis of Purified CD20 Mouse Monoclonal Antibody (IGEL/773). Confirmation of Purity and Integrity of Antibody.

CD20 / MS4A1 (B-Cell Marker) Antibody - Image 5

Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Clone
IGEL/773
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
MS4A1
Positive Control
and U266 cell lines. Human lymphocytes. Human lymph node or tonsil., Daudi, Raji
Immunogen
Recombinant full-length human MS4A1 protein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).
Key References
  • Tedder, T.F., et al. 1994. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol. Today 15: 450-454.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
931
SwissProt
P11836
Chromosome Location
11q12.2
Mol. Weight of Antigen
33-37kDa
Alternate Names
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, APY; ATOPY; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; Leukocyte surface antigen Leu-16; Ly44; MS4A1; MS4A2

Summary

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

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