2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> CBFB / PEBP2β

CBFB / PEBP2β

Mouse Monoclonal Antibody [Clone PCRP-CBFB-1E6]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Flow cytometric analysis of PFA-fixed HeLa cells. CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

img

SDS-PAGE Analysis Purified CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6). Confirmation of Purity and Integrity of Antibody.

img

Immunofluorescence Analysis of PFA-fixed U87 cells stained using CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

img

Western blot analysis of HeLa cell lysate using CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6).

img

Analysis of Protein Array containing more than 19,000 full-length human proteins using CBFB-Monospecific Mouse Monoclonal Antibody (PCRP-CBFB-1E6).

Product Details

Synonyms

CBF b; CBFB; Core-binding factor, beta subunit (CBFB), transcript variant 2; PEBP2 beta; PEBP2B; Polyomavirus enhancer binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit

Positive Control

HeLa, U87 or Jurkat cells.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IP, Flow Cyt, IF         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CBFB protein

Cellular Localization

Nucleus. Nucleoplasm. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a

Mol. Weight of Antigen

22kDa

Specificity & Comments

The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, and , which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2 subunits, PEBP2A, PEBP2B and PEBP2C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory subunit, which facilitates DNA binding of the subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2 is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2/CBFB-SMMHC, that is associated with human acute myeloid leukemia.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S, et al. Nature methods 15.5 (2018 May): 330-338.

Bioinformatics

  •  Entrez Gene ID: 865
  •  Gene Symbol: CBFB
  •  SwissProt: Q13951
  •  Chromosome Location: 16q22.1
  •  Unigene: 519632