Flow cytometric analysis of PFA-fixed HeLa cells. CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
SDS-PAGE Analysis Purified CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed U87 cells stained using CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Western blot analysis of HeLa cell lysate using CBFB Mouse Monoclonal Antibody (PCRP-CBFB-1E6).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CBFB-Monospecific Mouse Monoclonal Antibody (PCRP-CBFB-1E6).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, and , which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2 subunits, PEBP2A, PEBP2B and PEBP2C, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory subunit, which facilitates DNA binding of the subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2 is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle Myosin heavy chain, producing a chimeric gene, PEBP2/CBFB-SMMHC, that is associated with human acute myeloid leukemia.