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Home >> Antibodies >> Calprotectin / MRP14 / S100A9 / Calgranulin B

Calprotectin / MRP14 / S100A9 / Calgranulin B

Recombinant Mouse Monoclonal Antibody [Clone rMAC3781]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human spleen stained with S100A9 Recombinant Mouse Monoclonal Antibody (rMAC3781).

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Formalin-fixed, paraffin-embedded human spleen stained with S100A9 Recombinant Mouse Monoclonal Antibody (rMAC3781).

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SDS-PAGE Analysis Purified S100A9 Recombinant Mouse Monoclonal Antibody (rMAC3781). Confirmation of Integrity and Purity of Antibody

Product Details

Synonyms

60B8AG; Calgranulin A (CAGA); Calgranulin B (CAGB); Calprotectin L1L subunit; Chemotactic cytokine CP-10; Cystic fibrosis antigen (CFAG); Leukocyte L1 complex light chain; L1Ag; Macrophage L1 protein Calprotectin; Migration inhibitory factor related protein 8; Myeloid-related protein 8 (MRP-8 or P8); Myeloid-related protein 14 (MRP-14 or P14); Neutrophil cytosolic 7kDa protein; NIF

Positive Control

Tonsil, lymph node or spleen.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Affinity Purified monocyte membrane preparation

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Baboon. Monkey. Cow. Pig. Goat. Horse. Cat. Dog. Rabbit. Guinea Pig. Rat. Mouse.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

12-14kDa (doublet)

Specificity & Comments

Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer s cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Flavell DJ, et al. Journal of Histochemistry and Cytochemistry, 1987, 35(11):1217-

Bioinformatics

NBT03781