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Home >> Antibodies >> Calpastatin

Calpastatin

Mouse Monoclonal Antibody [Clone CAST/1550]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

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Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

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Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).

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SDS-PAGE Analysis Purified Calpastatin Mouse Monoclonal Antibody (CAST/1550). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

BS 17; Calpain inhibitor; Calpastatin; Cast; Heart type calpastatin; Sperm BS 17 component

Positive Control

HeLa cells. Esophagus or Small Intestine.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CAST protein

Cellular Localization

Cytoplasm and Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

126kDa

Specificity & Comments

Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Murachi, T. 1984. Calcium-dependent proteinases and specific inhibitors: calpain and calpastatin. Biochem. Soc. Symp. 45: 149-167.

Bioinformatics

  •  Entrez Gene ID: 831
  •  Gene Symbol: CAST
  •  SwissProt: P20810
  •  Chromosome Location: 5q15
  •  Unigene: 436186
NBT01550