Home >> Antibodies >> Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Recombinant Mouse Monoclonal Antibody [Clone rCALD1/820]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820).

Purified Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human ovary tissue lysate using Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820).

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820).

Purified Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of human ovary tissue lysate using Caldesmon Mouse Recombinant Monoclonal Antibody (rCALD1/820).

Product Details

Synonyms

CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22

Positive Control

Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Known Applications & Suggested Dilutions

Western Blot, Immunohistology More Details

Western Blot (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human CALD1 protein

Cellular Localization

Cytoplasmic

Species Reactivity

Human. Does not react with rat. Others not known.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

120-150kDa

Specificity & Comments

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.