Western Blot Analysis of MCF-7 & PC3 cell lysates using CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1).
Immunofluorescence Analysis of human MCF-7 cells labeling CHP2 with CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1) followed by Goat anti-mouse IgG-CF488 (Green).The nuclear counterstain is Reddot (Red)
SDS-PAGE Analysis Purified CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.