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Home >> Antibodies >> Calcineurin B homologous protein 2 / HCC Antigen 520

Calcineurin B homologous protein 2 / HCC Antigen 520

Mouse Monoclonal Antibody [Clone CPTC-CHP2-1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Western Blot Analysis of MCF-7 & PC3 cell lysates using CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1).

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Immunofluorescence Analysis of human MCF-7 cells labeling CHP2 with CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1) followed by Goat anti-mouse IgG-CF488 (Green).The nuclear counterstain is Reddot (Red)

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SDS-PAGE Analysis Purified CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

calcineurin-like EF hand protein 2; calcineurin-like EF-hand protein 2; HCA520; hepatocellular carcinoma antigen gene 520; Hepatocellular carcinoma-associated antigen 520 homolog

Positive Control

MCF-7, K562 or Jurkat cell lysates. Human appendix or testis.

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human full-length CHP2 protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2c, kappa

Mol. Weight of Antigen

22kDa

Specificity & Comments

CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ammar, YB. et al. The EMBO Journal, EMBO Press, 7 June 2006.

Bioinformatics

  •  Entrez Gene ID: 63928
  •  Gene Symbol: CHP2
  •  SwissProt: O43745
  •  Chromosome Location: 16p12.2
  •  Unigene: 178589
NBT02266