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Home >> Antibodies >> Calcineurin B / PPP3R1

Calcineurin B / PPP3R1

Mouse Monoclonal Antibody [Clone BE2.1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

calcineurin B, type I (19kDa); Calcineurin subunit B type 1; CALNB1; Cna2; CNB; CNB1; Ppp3r1; protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)

Positive Control

HeLa, MCF-7 or A431 cells. Human brain, breast or stomach tissue.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human Calcineurin B protein

Cellular Localization

Cytoplasmic.

Species Reactivity

Human. Xenopus laevis.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

19kDa

Specificity & Comments

Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.
  2. Zeng H. et al. (2001) Cell 107:617.

Bioinformatics

  •  Entrez Gene ID: 5534
  •  Gene Symbol: PPP3R1
  •  SwissProt: P63098
  •  Chromosome Location: 2p14
  •  Unigene: 280604
NBT02321