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Home >> Antibodies >> BOB.1 (B-Cell Marker)

BOB.1 (B-Cell Marker)

Mouse Monoclonal Antibody [Clone BOB1/2423]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2423).

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SDS-PAGE Analysis Purified BOB1 Mouse Monoclonal Antibody (BOB1/2423). Confirmation of Integrity and Purity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using BOB1 Mouse Monoclonal Antibody (BOB1/2423).

Product Details

Synonyms

B cell Oct binding protein 1; B-cell-specific coactivator OBF-1; BOB-1; OBF1; OCA-B; OCAB; OCT-binding factor 1; POU domain class 2-associating factor 1; POU2AF1

Positive Control

Ramos cells. Spleen or Tonsil.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

35kDa

Specificity & Comments

BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gibson SE, et al. Am J Clin Pathol. 2006 Dec;126(6):916-24.

Bioinformatics

  •  Entrez Gene ID: 5450
  •  Gene Symbol: POU2AF1
  •  SwissProt: Q16633
  •  Chromosome Location: 11q23.1
  •  Unigene: 654525
NBT02423