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BOB.1 (B-Cell Marker) Antibody [BOB1/7467]

Additional information

Catalog Number: 5450-MSM7
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5450-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5450-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5450-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
BOB.1 (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with BOB1 Mouse Monoclonal Antibody (BOB1/7467). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

BOB.1 (B-Cell Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human kidney in tonsil. Negative tissue control using BOB1/7467 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

BOB.1 (B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified BOB1 Mouse Monoclonal Antibody (BOB1/7467). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
BOB1/7467
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Nucleus.
Gene Name
POU2AF1
Positive Control
Raji or Ramos cells. Spleen or tonsil (IHC).
Immunogen
Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
Key References
  • Gibson SE, et al. Am J Clin Pathol. 2006 Dec;126(6):916-24.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5450
SwissProt
Q16633
Chromosome Location
11q23.1
Mol. Weight of Antigen
35kDa
Alternate Names
POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, B cell Oct binding protein 1; B-cell-specific coactivator OBF-1; BOB-1; OBF1; OCA-B; OCAB; OCT-binding factor 1; POU domain class 2-associating factor 1; POU2AF1

Summary

BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. �Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. �B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

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