Formalin-fixed, paraffin-embedded human tonsil stained with Biotinylated Lambda Light Chain probe followed by Anti-Biotin Mouse Monoclonal Antibody (Hyb-8).
SDS-PAGE Analysis Purified Anti-Biotin Mouse Monoclonal antibody (Hyb-8). Confirmation of Purity and Integrity of Antibody.
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.