2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Biotin (Vitamin B7 or Vitamin H)

Biotin (Vitamin B7 or Vitamin H)

Mouse Monoclonal Antibody [Clone Hyb-8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human tonsil stained with Biotinylated Lambda Light Chain probe followed by Anti-Biotin Mouse Monoclonal Antibody (Hyb-8).

img

SDS-PAGE Analysis Purified Anti-Biotin Mouse Monoclonal antibody (Hyb-8). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Vitamin B7 or Vitamin H

Positive Control

Biotinylated proteins in solution or on tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Confocal Microscopy
In situ Nucleic Acid Hybridization
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Biotinylated sheep immunoglobulin

Cellular Localization

Depends on the target

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

244Da

Specificity & Comments

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Uccellini MB,et al.2008.J. Immunol.181:5875.
  2. Green NM,et al.2012.J Biol Chem.287:39789

Bioinformatics