2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> Biotin (Vitamin B7 or Vitamin H)

Biotin (Vitamin B7 or Vitamin H)

Mouse Monoclonal Antibody [Clone Hyb-8]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
img

Formalin-fixed, paraffin-embedded human Tonsil stained with Biotinylated Lambda Light Chain probe followed by Anti-Biotin Mouse Monoclonal Antibody (Hyb-8).

img

SDS-PAGE Analysis of Purified anti-Biotin Mouse Monoclonal antibody (Hyb-8). Confirmation of Integrity and Purity of Antibody.

Product Details

Synonyms

Vitamin B7 or Vitamin H

Positive Control

Biotinylated proteins in solution or on tissues.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Confocal Microscopy
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
In situ Nucleic Acid Hybridization
Western Blot
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

Immunogen

Biotinylated sheep immunoglobulin

Cellular Localization

Depends on the target

Species Reactivity

All species

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

244Da

Specificity & Comments

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
  2. Green NM, et al. 2012. J Biol Chem. 287:39789

Bioinformatics

NBT00400