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Home >> Antibodies >> Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Beta-2 Microglobulin (Renal Failure & Tumor Marker)

Mouse Monoclonal Antibody [Clone C21.48A1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (C21.48A1). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of THP-1 and Raji cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (C21.48A1).

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Flow Cytometric Analysis of PFA-fixed HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (C21.48A1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

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Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (C21.48A1); followed by goat anti-mouse IgG-CF488 (Green).

Product Details

Synonyms

B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Positive Control

THP-1, RaJi, HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.

Known Applications & Suggested Dilutions

Flow Cyt, Western Blot, IF         More Details

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Soluble beta 2 microglobulin

Cellular Localization

Cell Surface and Extracellular (Secreted)

Species Reactivity

Human. Non-Human primates.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

12kDa

Specificity & Comments

The antibody recognizes the hidden determinant of beta-2 microglobulin (i.e. binding to its determinant is available only when the chain is separated from the HLA heavy chain. Beta-2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Liabeuf A, le Borgne de Kaouel C, Kourilsky FM, Malissen B, Manuel Y, Sanderson AR. An antigenic determinant of human beta 2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J Immunol. 1981 Oct;127(4):1542-8.

Bioinformatics

  •  Entrez Gene ID: 567
  •  Gene Symbol: B2M
  •  SwissProt: P61769
  •  Chromosome Location: 15q21-q22.2
  •  Unigene: 534255
NBT00433