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Home >> Antibodies >> Bcl-X (Apoptosis Marker)

Bcl-X (Apoptosis Marker)

Mouse Monoclonal Antibody [Clone SPM337]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bcl-x Mouse Monoclonal Antibody (SPM337).

Product Details

Synonyms

Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Positive Control

Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein

Cellular Localization

Cytoplasmic and cell/nuclear membrane

Species Reactivity

Human. Mouse. Rat. Pig.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

27kDa

Specificity & Comments

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
  2. Hsu YT, et. al. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(8):3668-72.
  3. Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.

Bioinformatics

  •  Entrez Gene ID: 598
  •  Gene Symbol: BCL2L1
  •  SwissProt: Q07817
  •  Chromosome Location: 20q11.21
  •  Unigene: 516966
NBT00751