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Home >> Antibodies >> BCL6

BCL6

MouseMonoclonal Antibody [Clone PCRP-BCL6-1E2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed HeLacells. BCL6 Mouse Monoclonal Antibody (PCRP-BCL6-1E2) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

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Immunofluorescence Analysis of MCF-7 cells using BCL6 Mouse Monoclonal Antibody (PCRP-BCL6-1E2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red)

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Analysis of Protein Array containing more than 19,000 full-length human proteins using BCL6Mouse Monoclonal Antibody (PCRP-BCL6-1E2).

Product Details

Synonyms

B-cell lymphoma 5 protein (BCL5); B-Cell Lymphoma 6 Protein; BCL6A; cys his2 zinc finger transcription factor; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S

Positive Control

HeLa or MCF-7 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human BCL6 protein

Cellular Localization

Nucleus. Mitochondria.

Species Reactivity

Human

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

95kDa

Specificity & Comments

Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Garc a JF,et al.2006.J. HistochemCytochem.54:31.
  2. Pasqualucci, L., et al. 2003. Leuk. Lymphoma 44 Suppl 3: S5-12.

Bioinformatics

  •  Entrez Gene ID: 604
  •  Gene Symbol: BCL6
  •  SwissProt: P41182
  •  Chromosome Location: 3q27.3
  •  Unigene: 478588