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Home >> Antibodies >> Alpha Actinin 4 / ACTN4

Alpha Actinin 4 / ACTN4

Mouse Monoclonal Antibody [Clone 93]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified ACTN4 Mouse Monoclonal Antibody (93). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

F-actin cross-linking protein; Focal segmental glomerulosclerosis 1 (FSG1); Non-muscle alpha-actinin-4

Positive Control

Human colon or kidney tissue (IHC).

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ACTN4 protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG

Mol. Weight of Antigen

105kDa

Specificity & Comments

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and -actinins. There are four tissue-specific -actinins, namely -actinin-1, -actinin-2, -actinin-3 and -actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each -actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding -actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Krusius, T. and Ruoslahti, E. 1986. Proc. Natl. Acad. Sci. USA 83: 7683-7687.

Bioinformatics

  •  Entrez Gene ID: 81
  •  Gene Symbol: ACTN4
  •  SwissProt: O43707
  •  Chromosome Location: 19q13.2
  •  Unigene: 270291
NBT03450