Formalin-fixed, paraffin-embedded human pancreas stained with AKT1 Mouse Monoclonal Antibody (AKT1/2784). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human pancreas stained with AKT1 Mouse Monoclonal Antibody (AKT1/2784).
Western Blot Analysis of human NIH3T3 cell lysate using AKT1 Mouse Monoclonal Antibody (AKT1/2784).
SDS-PAGE Analysis Purified AKT1 Mouse Monoclonal Antibody (AKT1/2784). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using AKT1-Monospecific Mouse Monoclonal Antibody (AKT1/2784).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB or RacPK-) and Akt 3 (also designated PKB or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR- tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.