|Catalog No||Formulation||Size||Price (USD)|
|207-MSM2-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|207-MSM2-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||429.00|
|207-MSM2-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||429.00|
|Human Entrez Gene ID||207|
|Human Gene Symbol||AKT1|
|Human Chromosome Location||14q32.32|
|Synonyms||AKT1; Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha|
|Immunogen||Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||62kDa|
|Cellular Localization||Nuclear, Cytoplasmic and Cell Surface|
|Species Reactivity||Human. Others-not known.|
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB or RacPK-) and Akt 3 (also designated PKB or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR- tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.