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Home >> Antibodies >> AKT1 (Prognostic Marker for Neuroendocrine Tumors)
AKT1 (Prognostic Marker for Neuroendocrine Tumors)

Mouse Monoclonal Antibody [Clone AKT1/2552]

Catalog #
Size
20 ug
100 ug
100 ug
Price (USD)
199.00
429.00
429.00
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Catalog No
Size
20 ug
100 ug
100 ug
Formulation
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Price (USD)
199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Pancreas stained with AKT1 Mouse Monoclonal Antibody (AKT1/2552).

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SDS-PAGE Analysis Purified AKT1 Mouse Monoclonal Antibody (AKT1/2552). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human HeLa cell lysate using AKT1 Mouse Monoclonal Antibody (AKT1/2552).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using AKT1 Mouse Monoclonal Antibody (AKT1/2552).

Product Details

Synonyms

AKT1; Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha

Positive Control

PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)

Cellular Localization

Nuclear, Cytoplasmic and Cell Surface

Species Reactivity

Human. Others-not known.

Host / Ig Isotype

Mouse / IgG2b, kappa

Specificity & Comments

Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB or RacPK-) and Akt 3 (also designated PKB or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR- tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

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200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Franke, T.F., et al. 1995. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81: 727-736.

Bioinformatics

  •  Entrez Gene ID: 207
  •  Gene Symbol: AKT1
  •  SwissProt: P31749
  •  Chromosome Location: 14q32.32
  •  Unigene: 525622