SPI-C / Transcription factor Spi-C [PCRP-SPIC-2C5]

Clear

Summary

The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65% amino acid identity with its mouse counterpart, suggesting a conserved role between species.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Nucleus
Gene Name
Positive Control
Jurkat or Raji cells.
Immunogen
Recombinant full-length human SPIC protein
Alternate Names
Transcription factor Spi-C, AU019198; C76795; MGC40611; MGC80555; Prf; Pu.1 related factor; Spi C; Spi C transcription factor; Spi-C transcription factor (Spi-1/PU.1 related)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
PCRP-SPIC-2C5
Human Gene Symbol
SPIC
Chromosome Location
12q23.2
Mol. Weight of Antigen
29kDa

Functions

  • Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression (By similarity).

Known Applications & Suggested Dilutions

  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • ,Optimal dilution for a specific application should be determined.

Key References

  • Carlsson, R., et al. 2002. Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. Gene 299: 271-278.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “SPI-C / Transcription factor Spi-C”

Your email address will not be published. Required fields are marked *

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies