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Recombinant Crystallin Alpha B / CRYAB (Marker of Metaplastic Carcinoma) Antibody [rG2JF]

In Stock
Catalog Number Formulation Size Price
1410-MSM23-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
1410-MSM23-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
1410-MSM23-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Alpha B-crystallin Antibody in Immunohistochemistry (IHC (P))

Alpha B-crystallin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human kidney stained with Recombinant Alpha B-crystallin Mouse Monoclonal Antibody (rG2JF). Inset: PBS instead of primary antibody; secondary only negative control.

Alpha B-crystallin Antibody in SDS-PAGE

Alpha B-crystallin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Recombinant Alpha B-crystallin Mouse Monoclonal Antibody (rG2JF). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Alpha B crystallin is a lens protein which has some homology with the small heat shock proteins. It is expressed in tissues such as skeletal muscle, cardiac muscle, smooth muscle, renal tubular epithelium, Schwann cells, glial cells, thyroid epithelium, colonic epithelium and stratified squamous epithelium. Alpha B crystallin is also reported to be found in ubiquitinated intermediate filament inclusion bodies, such as Lewy bodies (neurofilaments), Rosenthal fibres (glial filaments) and Mallory bodies (cytokeratins). Alpha B crystallin is rarely found in neurofibrillary tangles. The role of Alpha B crystallin in these inclusion bodies is unknown, however, it may function as an accessory protein for intermediate filament aggregation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
CRYAB
Positive Control
Human heart, kidney or brain.
Immunogen
Synthetic peptide hemocyanin conjugate. The peptide corresponds to human alpha B crystallin amino acids 1 to 10.
Alternate Names
Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, AACRYA; Alpha B crystallin; Alpha-crystallin B chain; CRYA2; CTTP2; Heat shock 20 kD like protein; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY REN 27; Rosenthal fiber component

Database Links

Entrez Gene ID
1410
SwissProt
P02511

Additional Information

Clone
rG2JF
Chromosome Location
11q23.1
Mol. Weight of Antigen
Predicted: 20kDa; Observed: 22-30kDa

Related Antibodies

View All CRYAB Antibodies

Functions

  • May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

Key References

  • Haley, D.A., et al. 1998. J. Mol. Biol. 277: 27
  • Leach I H, Tsang M L, Church R J, et al.. Journal of Pathology. 173: 255–260 (1994).
  • Pinder, S E et al. “The expression of alpha B-crystallin in epithelial tumours: a useful tumour marker?.” The Journal of pathology vol. 174,3 (1994): 209-15. doi:10.1002/path.1711740310.
  • Lowe, J et al. “alpha B crystallin expression in non-lenticular tissues and selective presence in ubiquitinated inclusion bodies in human disease.” The Journal of pathology vol. 166,1 (1992): 61-8. doi:10.1002/path.1711660110
  • Iwaki, T et al. “Cellular distribution of alpha B-crystallin in non-lenticular tissues.” The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society vol. 38,1 (1990): 31-9. doi:10.1177/38.1.2294148
  • Lowe, J et al. “Dementia with beta-amyloid deposition: involvement of alpha B-crystallin supports two main diseases.” Lancet (London, England) vol. 336,8713 (1990): 515-6. doi:10.1016/0140-6736(90)92075-s

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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