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Crystallin Alpha B Antibody [CRYAB/7918]

In Stock
Catalog Number Formulation Size Price
1410-MSM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1410-MSM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1410-MSM18-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into α, β and γ families, and the β- and γ-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide and N- and C-terminal extensions. α-crystallins consist of three gene products, αA-, αB- and αC-crystallin, which are members of the small heat shock protein family (HSP 20). α-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, α-crystallins do not renature these proteins. Expression of αA-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human αB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of αB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
Positive Control
Human heart, kidney or brain. HeLa or Y79 cells.
Immunogen
Recombinant human full-length CRYAB protein
Alternate Names
Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, AACRYA; Alpha B crystallin; Alpha-crystallin B chain; CRYA2; CTTP2; Heat shock 20 kD like protein; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY REN 27; Rosenthal fiber component

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
CRYAB/7918
Chromosome Location
11q23.1
Mol. Weight of Antigen
Predicted: 20kDa; Observed: 22-30kDa

Functions

  • May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

Key References

  • Haley, D.A., et al. 1998. J. Mol. Biol. 277: 27

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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