Recombinant PDCD1 / PD1 / CD279 (Programmed Cell Death 1) [PDCD1/4231R]

Clear

Summary

PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG, kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
PDCD1
Positive Control
Human tonsil|lymph node or appendix.
Immunogen
Recombinant fragment corresponding to extracellular domain of human PDCD1 protein (exact sequence is proprietary)
Alternate Names
Programmed cell death protein 1, CD279; hPD-1; hSLE1; PD1; PDCD1; Programmed Cell Death Protein 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2

Database Links

Entrez Gene ID
5133
Unigene
158297
SwissProt
Q15116

Additional Information

Clone
PDCD1/4231R
Human Gene Symbol
PDCD1
Chromosome Location
2q37.3
Mol. Weight of Antigen
55kDa

Functions

  • Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).
  • The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).

Known Applications & Suggested Dilutions

  • ELISA (For coating, order antibody without BSA)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Roncador, G., et al. Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma. Haematologica. 2007 Aug;92(8):1059-66.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “PDCD1 / PD1 / CD279 (Programmed Cell Death 1)”

Your email address will not be published. Required fields are marked *

Related

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Resources
Newsletter Signup NeoBiotechnologies
Updates & Sales

$0.00
510-376-5603