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SDS-PAGE Analysis of Purified KLF12 Mouse Monoclonal Antibody (PCRP-KLF12-1E3). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence analysis of PFA-fixed HeLa cells. KLF12 Mouse Monoclonal Antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (green).
Flow cytometric analysis of PFA-fixed HeLa cells. KLF12 Mouse Monoclonal Antibody (PCRP-KLF12-1E3) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using KLF12 Mouse Monoclonal Antibody (PCRP-KLF12-1E3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.
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