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GTF2H2 / BTF2 / TFIIH Basal Transcription Factor Antibody [PCRP-GTF2H2-1B9]

In Stock
Catalog Number Formulation Size Price
2966-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2966-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2966-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-2ug/ml

Summary

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and Mat1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or MCF7 cells.
Immunogen
Recombinant full-length human GTF2H2 protein
Alternate Names
General transcription factor IIH subunit 2, Basic transcription factor 2 44 kDa subunit, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit, Basic transcription factor 2 44 kDa subunit; BTF2; BTF2-p44; BTF2P44; General transcription factor IIH, polypeptide 2, 44kDa; T-BTF2P44; TFIIH basal transcription factor complex p44 subunit

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-GTF2H2-1B9
Chromosome Location
5q13.2
Mol. Weight of Antigen
44kDa

Functions

  • Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.

Key References

  • Conaway, R.C., et al. 1989. Proc. Natl. Acad. Sci. USA 86: 7356-7360.
  • Weeda, G., et al. 1990. Cell 62: 777-791.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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