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Recombinant EpCAM / CD326 (Epithelial Marker) Antibody [rEGP40/4547]

In Stock
Catalog Number Formulation Size Price
4072-MSM27-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4072-MSM27-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4072-MSM27-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant EpCAM / CD326 (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/4547).

Recombinant EpCAM / CD326 (Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/4547).

Recombinant EpCAM / CD326 (Epithelial Marker) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rEGP40/4547 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant EpCAM / CD326 (Epithelial Marker) Antibody - Image 4

SDS-PAGE Analysis of Purified EpCAM Recombinant Mouse Monoclonal Antibody (rEGP40/4547). Confirmation of Purity and Integrity of Antibody.

EpCAM Antibody in Western Blot (WB)

EpCAM Antibody in Western Blot (WB)

Western blot analysis of HCT116 and HEK293 lysates using EpCAM Mouse Recombinant Monoclonal Antibody (rEGP40/4547).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Stem Cell Differentiation
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
HT29 cells. Human breast tumor. HCT116, HEK293.
Immunogen
Recombinant full-length human EpCAM protein
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1); ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1); REG-1; Reggie-1; Reggie-2

Database Links

Entrez Gene ID
4072
SwissProt
P16422

Additional Information

Clone
rEGP40/4547
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa

Related Antibodies

View All EPCAM Antibodies

Functions

  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Key References

  • Postmus PE, et al. 1986. Cancer. 57:60-3.
  • Manon J. Winter,et al. J Biol Chem 125:437-446 (1994).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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